路德社4/3/2021路德時評(路博艾談):HBO王牌脫口秀主持人Bill Maher節目都開始談論的話題太重磅了意味著什麼? 訪談開始點出了Dr. Lawrence [email protected]上午1:03 · 2021年4月4日·Twitter Web App發佈的推文——

The #COVID19 virus was likely pre-adapted for human infection by serial passage through “humanized” animal models To explore such capabilities, begin here #COVID #Corona #coronavirus #DRASTIC #coronavirus #CCPVirus #UnrestrictedBiowarfare #UnrestrictedBioweapon #OriginOfCOVID19


那麼Dr. Lawrence Sellin到底是什麼人呢?這則推文又意味著什麼呢?據路德社4/3/2021路德時評(路博艾談):HBO王牌脫口秀主持人Bill Maher節目都開始談論的話題太重磅了意味著什麼?時間點25:52

[Larence Sellin博士是美國前軍情部門專家,他的推是零FOLLOW,從來不FOLLOW任何人,這個推就是純粹傳遞資訊的,他發的是什麼東西?今天發的這個東西絕對重磅,他說COVID19病毒很可能是通過提前適應了讓人類感染被一系列的“人性化”的動物模型通過連續傳代感染,然後具備傳染人的能力,從這裡開始——超限生化武器/冠狀病毒的來源。然後附上鄧宏魁和秦川的照片,這個人絕對是軍情部門的,他是生化武器專家,第一次提到動物傳達,我們在119第一次提到動物傳代,閆博士告訴大家通過動物傳代打磨,然後她在第一份報告裡頭,閆博士的第一份報告裡面就講了一整頁,第19頁第五部分大家去看。]


Step 5: Optimize the virus for fitness and improve its hACE2-binding affinity in vivo (2.5-3 months) 步驟5:優化病毒的適應性,提⾼病毒在體內的hACE2結合親和⼒(2.5-3個⽉)

Virus recovered from step 4 needs to be further adapted undergoing the classic experiment – serial passage in laboratory animals101. This final step would validate the virus’ fitness and ensure its receptororiented adaptation toward its intended host, which, according to the analyses above, should be human. Importantly, the RBM and the furin-cleavage site, which were introduced into the Spike protein separately, would now be optimized together as one functional unit. Among various available animal models (e.g. mice, hamsters, ferrets, and monkeys) for coronaviruses, hACE2 transgenic mice (hACE2-mice) should be the most proper and convenient choice here. This animal model has been established during the study of SARS-CoV and has been available in the Jackson Laboratory for many years102-104.


The procedure of serial passage is straightforward. Briefly, the selected viral strain from step 4, a precursor of SARS-CoV-2, would be intranasally inoculated into a group of anaesthetized hACE2-mice. Around 2-3 days post infection, the virus in lungs would usually amplify to a peak titer. The mice would then be sacrificed and the lungs homogenized. Usually, the mouse-lung supernatant, which carries the highest viral load, would be used to extract the candidate virus for the next round of passage. After approximately 10~15 rounds of passage, the hACE2-binding affinity, the infection efficiency, and the lethality of the viral strain would be sufficiently enhanced and the viral genome stabilized101. Finally, after a series of characterization experiments (e.g. viral kinetics assay, antibodies response assay, symptom observation and pathology examination), the final product, SARS-CoV-2, would be obtained, concluding the whole creation process. From this point on, this viral pathogen could be amplified (most probably using Vero E6 cells) and produced routinely.


It is noteworthy that, based on the work done on SARS-CoV, the hACE2-mice, although suitable for SARS-CoV-2 adaptation, is not a good model to reflect the virus’ transmissibility and associated clinical symptoms in humans. We believe that those scientists might not have used a proper animal model (such as the golden Syrian hamster) for testing the transmissibility of SARS-CoV-2 before the outbreak of COVID-19. If they had done this experiment with a proper animal model, the highly contagious nature of SARS-CoV-2 would be extremely evident and consequently SARS-CoV-2 would not have been described as “not causing human-to-human transmission” at the start of the outbreak.


We also speculate that the extensive laboratory-adaptation, which is oriented toward enhanced transmissibility and lethality, may have driven the virus too far. As a result, SARS-CoV-2 might have lost the capacity to attenuate on both transmissibility and lethality during its current adaptation in the human population. This hypothesis is consistent with the lack of apparent attenuation of SARS-CoV-2 so far despite its great prevalence and with the observation that a recently emerged, predominant variant only shows improved transmissibility105-108.


Serial passage is a quick and intensive process, where the adaptation of the virus is accelerated. Although intended to mimic natural evolution, serial passage is much more limited in both time and scale. As a result, less random mutations would be expected in serial passage than in natural evolution. This is particularly true for conserved viral proteins, such as the E protein. Critical in viral replication, the E protein is a determinant of virulence and engineering of it may render SARS-CoV-2 attenuated109-111 Therefore, at the initial assembly stage, these scientists might have decided to keep the amino acid sequence of the E protein unchanged from that of ZC45/ZXC21. Due to the conserved nature of the E protein and the limitations of serial passage, no amino acid mutation actually occurred, resulting in a 100% sequence identity on the E protein between SARS-CoV-2 and ZC45/ZXC21. The same could have happened to the marks of molecular cloning (restriction sites flanking the RBM). Serial passage, which should have partially naturalized the SARS-CoV-2 genome, might not have removed all signs of artificial manipulation.



綜述:我們大部分人都不是中共病毒專家,所以即使把閆麗夢博士的報告放在我們面前,我們也無法徹底讀懂它,但是閆麗夢博士的報告正在喚醒這個世界,美國軍情生化武器專家Larence Sellin博士已經通過推文公開認可了閆麗夢博士關於中共病毒是[超限生化武器]的定義和定性,並且Larence Sellin博士已經在推文中列出中共的超限生化武器專家名單,我相信這份名單要甚于當年美軍在伊拉克公佈的伊拉克前政要人員撲克牌,我相信凡是上這個榜單的所有中共專家他們都無法逃避最終的審判!


0 則留言
Inline Feedbacks
View all comments


4月 04日