Communist China Uses State Funds to Support Top Scientists to Create New Cross-Species Transmissible, Highly Infectious, Highly Pathogenic Viruses

Document Translation: GBW, WMSky

Preface: WMSky | Proofread: GBW

——The original three-year research content of the 2011 funding application for the National Foundation of China by Gao Fu, Institute of Microbiology, Chinese Academy of Sciences

Foreword

Lude Media’s February 20, 2021 evening program told viewers that someone had unearthed a funding application submitted in 2011 to the Chinese National Fund by Gao Fu, chief scientist of the Chinese Academy of Sciences and now director of the Chinese Center for Disease Control and Prevention (CDC of China). Judging from the title of the document shown in the program, it was a major five-year research project devoted to the study of so-called “important viruses” that cause interspecies infectious diseases and their mechanism.

In the program, Lude showed the viewers three of the pages of the application, i.e., the research content and objectives for the first three years (see below). The research project began with collecting a large number of virus samples across the country, from different regions and animals, including wildlife, zoo animals and special breeding animals (bats, rodents, insectivores), and domestic animals (chickens, pigs and dogs). Of these, 500 were bat samples and 500 were domestic animal samples, for a total of up to 1,000!  The viruses involved were influenza virus, novel coronavirus, hantavirus, encephalitis B virus, dengue virus, rabies virus, and West Nile virus. The research goal is to use modern biotechnological means to obtain new viruses that are highly infectious, highly pathogenic and especially neurophilic (able to infect nerve cells).

“The content is very scary!” screamed Lude, the host of Lude Media.” This is consistent with the description of the new concept of ‘Contemporary Genetic Weapons’ in the Chinese Communist military textbooks and with the concept of Unrestricted Biological Weapons that Dr. Yan Limeng told us about in this program.” 

The military textbook referred to by Luther, titled “Unnatural Origins of SARS-CoV and New Man-Made Human Virus as Genetic Weapons,” was published by the Chinese Military Medical Science Press in 2015. Its English version is in the process of being jointly translated by the Whistleblower’s Movement worldwide and will be available soon.

One wonders, why was the CCP doing this? What was this for? Apparently, this study did not prevent the SARS-CoV-2 outbreak. Curious to know what role its findings played in the pandemic of the century in which we are in the midst of at this moment?

Fig.1 The three pages of the application showed in Lude Media program

Project Name: Study on the molecular mechanism of important viruses’ cross-species infection and transmission

Chief Scientist:Gao Fu(高福), Institute of Microbiology, Chinese Academy of Sciences 

Starting and ending years:Jan 2011 to Aug 2015

Dependent department: Chinese Academy of Sciences

1st year Research Content and Expected Objectives

Research Content

  1. To collect a large number of samples of serum, pharyngeal and anal swab from bats, rodents, and insectivores throughout the country. To collect cotton swabs, tissue, and blood samples from different regions and animals, including wild animals, zoo animals, and special animals farmed for profit, and livestock and poultry including chickens, pigs, and dogs.
  2. To conduct epidemiological studies on the isolation and viral epidemiology of influenza viruses, novel coronaviruses, and encephalitis B viruses, etc. To study the host characteristics of viruses and to perform genome-wide determination and analysis of isolated and identified viruses. To observe the biological characteristics of novel viruses, with emphasis on the pathogenicity and transmissibility in animals, and the mechanisms of replication and cross-species transmission of influenza viruses in different hosts.
  3. To utilize prokaryotic or eukaryotic expressions on the envelope proteins of encephalitis B virus, dengue virus, and influenza virus. To use molecular sieve chromatography, ion exchange, and other techniques to separate and purify proteins. To carry out crystal screening on viral envelope protein; to establish cell models for autophagy pathway studies.
  4. Mechanisms of replication and cross-species transmission of coronavirus in different hosts; mechanisms of replication and cross-species transmission of dengue viruses in different hosts; study of regulatory and molecular mechanisms of viral replication through expression profiling of miRNA replicated by viruses in different tissues. 
  5. Based on the well-established viral protein and host factor interaction platform, to search for the host restriction factors of influenza virus; to screen the host cell factors that interact with the main protein of hantavirus (nucleoprotein). By means of protein interaction, to identify host-related proteins that differ between species strains and obtain immune-related cells and central nervous system tissue material from early latency and mid-and late-infection stages of infection with rabies virus strains of different virulence.
  6. Construction of human MHC transgenic mice; identification of the characteristics of neutrophilic biology of the novel viruses such as highly pathogenic avian influenza virus and West Nile virus.
  • Expected Objectives
  1. To collect 500 bat samples; 500 samples of domestic animals.
  2. To obtain isolated strains and sequences of influenza virus, new coronavirus, encephalitis B virus, etc.; to obtain the pathogenicity and transmission characteristics of the isolated strains.
  3. To obtain the envelope proteins of different genotypes of encephalitis B virus, dengue virus, and influenza virus; to obtain the crystal structures of some envelope proteins.
  4. To perform targeted mutation on the target gene of the virus, observe the difference in replication ability of the virus mutant strain and the wild strain in different host cells and cells of different tissue sources, and find the key sites that determine virus replication in host cells of different species.
  5. To screen for 5-10 host cytokines that interact with the viral proteins of influenza virus, hantavirus, and rabies virus, respectively.
  6. To obtain human HLA-A2/DR1 and HLA-Al11/DR1 transgenic mice. To complete the biological characterization of 5-10 novel viruses such as highly pathogenic avian influenza virus and rabies virus and obtain at least 4 novel viruses with different neurotropism.
  7. To publish more than 10 research papers.

 2nd year Research Content and Expected Objectives

  • Research Content
  1. To detect novel coronaviruses, novel hantaviruses, novel paramyxoviruses, and novel influenza viruses in animals; virus culture and isolation of virus-positive samples; analysis of differences and evolutionary relationships between the newly detected virus sequences and their known human counterparts.
  2. To continue to isolate and investigate viruses; to study the characteristics and patterns of changes in the biological properties of viruses after transmission through different hosts; to conduct whole-genome determination of isolated and identified viruses, use genome informatics analysis techniques to study the genetic variation and molecular evolution patterns and characteristics of viruses, and establish a reverse genetic manipulation technology for viruses.
  3. To isolate virus-susceptible cell membrane proteins and screen candidate receptors using GST pull-down; to identify candidate receptors by mass spectrometry; study of the interaction characteristics of different influenza virus HA proteins with receptors; to study the molecular mechanism of autophagy affecting influenza virus invasion on cell models.
  4. To study the replication and cross-species transmission mechanism of influenza virus in different hosts; the replication and cross-species transmission mechanism of coronaviruses in different hosts; the replication and cross-species transmission mechanism of dengue virus in different hosts; To study the regulatory and molecular mechanisms of viral replication through gene expression profiling of miRNA replicated by viruses in different tissues. 
  5. To explore the regulatory mechanisms of host restriction factors in the replication of influenza viruses using protein-protein interactions and virological methods. To track the influence and biological significance of the interaction between host restriction factors and viral nucleoprotein on virus replication, assembly, and mature release. To identify differences at the proteomic level exhibited by the host before and after rabies virus infection using proteomic analysis of peripheral immune-related cells and central nervous tissue by chromatographic mass spectrometry.
  6. To establish an animal model for real-time monitoring of virus distribution in vivo and conduct a preliminary exploration on the infection kinetics of highly pathogenic avian influenza viruses and other viruses.
  • Expected Objectives
  1. To obtain partial genome sequences of more than 10 viruses; to determine the serological and genetic sequence differences between new viruses and human viruses.
  2. To obtain isolated strains and whole-genome sequences of influenza virus, new coronavirus, and encephalitis B virus; to obtain the genetic variation patterns and biological properties of the prevalent strains; to establish 1 set of reverse genetic manipulation system of viruses.
  3. To obtain cellular candidate receptors for encephalitis B virus and dengue viruses; to obtain some important protein residues affecting influenza virus HA protein and sialic acid receptor; to gain a preliminary understanding of how influenza virus affects the signaling pathway of cellular autophagy.
  4. To obtain target recombinant viruses; to establish a human coronavirus library to reveal the effects of different mutations on viral RNA translation and replication; to obtain host cell miRNA and viral miRNA expression profiles.
  5. To screen 2-6 host restriction factors that interact with influenza viruses and hantaviruses, respectively; to explain the effects of some host restriction factors on the replication of influenza viruses and hantaviruses and their mechanisms of action; to obtain the differences in protein levels of the host before and after rabies infection.
  6. To clarify the infection characteristics of highly pathogenic avian influenza viruses in HLA-A2/DR1 and HLA-A11/DR1 transgenic mice, and to preliminarily reveal the role of MHC restriction in process of cross-species infection of highly pathogenic avian influenza viruses; to complete the genome sequencing of the new viruses, use bioinformatics methods, and discover neurotropism-related virulence loci, and verify them by reverse genetics.
  7. To publish more than 20 research papers.

3rd year Research Content and Expected Objectives

  • Research Content
  1. To perform virus isolation and culture for those that test positive for virulence; to conduct cell and animal sensitivity experiments of newly classified viruses to evaluate the host range and pathogenicity of viruses in experimental animals. 
  2. To continue investigations and studies on molecular epidemiology and analyze the evolution and mutation patterns of viruses; using the reverse genetic manipulation technology platform to study the effects of viral point mutations, gene mutations, and gene reassortment on viral pathogenicity and transmission, with emphasis on the key factors or factors affecting the viral transmission characteristics, especially the characteristics of cross-species transmission. 
  3. Further validation of screened candidate receptors for encephalitis B viruses and dengue viruses on cellular models; expression purification of influenza virus mutants, the crystal structure of complexes with sialic acid receptors; study of the molecular mechanism of autophagy affecting influenza virus invasion on cellular models.
  4. To study the replication and cross-species transmission mechanism of influenza virus in different hosts; the replication and cross-species transmission mechanism of coronaviruses in different hosts; the replication and cross-species transmission mechanism of dengue virus in different hosts; To study the regulatory and molecular mechanisms of viral replication through gene expression profiling of miRNA replicated by viruses in different tissues. 
  5. To continue to explore the mechanisms of host restriction factors in the regulation of influenza virus and hantavirus replication. To explore the nature of weak virus-induced early anti-infection factors (host restriction factors) and the effects of strong virus-induced early pathogenic factors on host restriction factors.
  6. To construct an animal model for real-time monitoring of virus distribution in vivo; to conduct functional studies on the neurotropic-related virulence loci of the new viruses.
  • Expected Objectives
  1. To obtain the complete genome sequence of 3-5 newly isolated viruses; to reveal the genetic variation in wildlife or domestic animal coronaviruses. 
  2. To establish virulent strain libraries and gene banks; to identify key gene loci that affect viral pathogenicity and transmissibility.
  3. To obtain 1-2 receptors or co-receptors for encephalitis B viruses and dengue viruses; to reveal the mechanism by which the interaction between influenza virus HA protein and sialic acid receptor affects the cross-species transmission of influenza viruses at the molecular structural level. To show how the express proteins of the influenza virus affect the level of cellular autophagy.
  4. To reveal the dynamics of virus growth ability in different hosts; to obtain infectious clones of human coronaviruses; to obtain cellular protein factors on four viral RNAs.
  5. To show the regulatory mechanisms of host restriction factors in the replication of influenza virus and hantavirus; to screen 1-2 host restriction factors that interact with rabies viruses.
  6. To obtain an animal model for real-time monitoring of influenza virus distribution in vivo by transgenic technology; to show preliminarily the mechanism of action of the neurotropic-related virulence loci of the new virus through cytological and zoological experiments. To publish more than 20 research papers.

Information source: 

  1. Lude Media Evening Show, February 20, 2021 https://www.youtube.com/watch?v=-HucSkjWxjs&t=3190s
  2. 中科院微生物研究所高福2011年申请中国国家基金的资金申请书三年研究内容原文陈列 ——“重要病毒跨种间感染与传播致病的分子机制研究”, https://gnews.org/zh-hans/935149/

Reviewer: Helen

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