DT excavator notes:
In the previous article, we exposed the factual clues that the CCP used the state apparatus to kidnap 1.4 billion Chinese people to steal US scientific and technological achievements and talents. We also explained the implementation method and process of the “BGY” plan through several case studies. It can be said that through various BGY means, they almost achieved their purpose of 3F America.
At the heart of this shipwreck program against the United States is the development of “Viral Biochemical Weapons”. Is the novel coronavirus the only weapon in the plan? Absolutely not. The CCP organized a huge scientific research team, established two P4 safety level biological laboratories and many P3 level biological safety laboratories. This means that they prepared a huge strategic reserve, the purpose is not to develop a weapon, but many more biochemical weapons.
The following are the details:
We will first have a look at ‘Champion’s Daddy’s series of tweets. These tweets analyzed what viruses are being collected and possibly being used for bioweapon research by looking at scientific papers.
This long tweet reveals that, in addition to the coronavirus, the CCP P4 laboratory has a collection of viral pathogens.
The CCP coronavirus outbreak in 2019 has shown the world that the P4 laboratories are the bioweapons manufacturing center for the CCP.
This tweet selected 15 representative published papers to reveal what viruses were collected by the P4 laboratories of the CCP.
Now that the coronavirus has been used as a biochemical weapon, other viruses may also be used for the same purpose.
The first paper was published in Journal of GENERAL VIROLOGY in 2011. A new Hantavirus was found in voles in Yunnan province.
Hantavirus is a single-stranded RNA virus, which can produce hantavirus disease after being transmitted to humans through mice, and is classified as a level 4 biological hazard. The virus can cause hemorrhagic fever and severe kidney damage. The 2013-2018 outbreak in Argentina had a mortality rate of 18.6% with some areas reaching 40%s.
The second paper was published in JOURNAL OF GENERAL VIROLOGY in 2011
A new cyclic single-stranded DNA virus has been discovered in bats.
It is not clear what disease this virus causes in humans. The circovirus genus of virus, known to infect pigs, can cause dermatitis, nephropathy and reproductive disease in pigs.
The third paper was published in JOURNAL OF GENERAL VIROLOGY in 2011
This paper is about the discovery that mutations in the receptor protein on the surface of human cells can make a deadly virus, Nipah virus, 30 times more capable of entering cells.
This paper is about the discovery of mutations in the receptor protein on the surface of human cells can make a deadly virus, Nipah virus, 30 times more capable of entering cells
Nipah virus is an RNA virus that causes respiratory diseases and encephalitis.
WHO estimates that the mortality rate is 40-75%.
The fourth paper was published in VIROLOGY JOURNAL in 2012 without direct isolation of the virus.
This paper says although Ebola hasn’t been isolated from bats, the evidence of Ebola in bats is found.
Ebola virus will be explained in detail later.
5. The fifth paper
This paper was published in Journal of General Virology in 2014
Novel astrovirus has been detected in 39 different animals, most of which are bats and rodents. This paper concerned only testing but not isolation of the virus.
Novel astrovirus has been detected in 39 different animals, most of which are bats and rodents. This paper concerned only testing but not isolation of the virus.
6. Paper Six
Published in JOURNAL OF GENERAL VIROLOGY in 2015
This article is about a virus isolated from bats that is very similar to the orthoreovirus in human and pigs
This virus is a double-stranded RNA virus, which normally causes no symptoms. In some cases it can also cause pneumonia and respiratory diseases.
7. Paper Seven
Published in JOURNAL OF VIROLOGY in 2015
Novel coronavirus isolated from bat claimed to be close to SARS
Not much to say about this, we are all familiar with the coronavirus.
8. Paper Eight
Posted in JOURNAL OF GENERAL VIROLOGY 2016
Novel adenovirus isolated in bat
Adenovirus is a double-stranded DNA virus, and infection does not normally cause symptoms. But for some people with defective immune systems, such as those catheterised, infection can cause symptoms such as pneumonia, hepatitis, and cystitis
This virus is widely used as a carrier virus in biology because it does not cause symptoms.
9. Paper Nine
It’s still adenovirus, but this time it’s isolated from a monkey, not a bat.
Published in VIROLOGY JOURNAL in 2016
10. Paper Ten
Published in VIROLOGY JOURNAL in 2016
A hantavirus has been detected in some kind of mouse in Yunnan.
What is Hantavirus? Please see the analysis of the first paper
This was done in cooperation with a disease center in Yunnan
11. Paper Eleven
Published in VIROLOGICA SINICA in 2017
It says that novel astroviruses, caliciviruses and rotaviruses are found in bats.
This article only mentions the viruses are detected, but not separated, so these viruses will not be discussed much further. Those who are interested can perform their own search for these viruses.
12. Paper Twelve
Published in VIROLOGICAL JOURNAL in 2017
This article is about collecting the livers of dead bats to test for viruses and finding new hepatitis viruses in some of them.
Does the hepatitis virus need more introduction? Hepatitis B and Hepatitis C virus are all hepadnaviruses
13. Paper Thirteen
This article was published in VIROLOGICA SINICA in 2017
Nairovirus isolated from fleas can cause Crimean-Congo haemorrhagic fever.
It is a highly contagious RNA virus, similar to Hantavirus, with a mortality rate of about 25%
14. Paper Fourteen
This paper was published in the Journal of Virology in 2018
A novel bat coronavirus that uses the same receptor as the MERS virus was isolated from the bat
MERS is the coronavirus that caused the Middle East Respiratory Syndrome in 2015, much like SARS
15. Paper Fifteen
This paper was published in NATURE MICROBIOLOGY in 2019. It is one that we need to focus on a bit.
This paper says that a filamentous virus is isolated from bats, which is between Ebola virus and Marburg virus. It can infect people with the same cell surface receptors as Ebola virus, which is basically a bat version of Ebola.
16. Ebola virus and Marburg virus are infamous.
The WHO description of Ebola virus and Marburg virus is shown in the figures below.
These two viruses are the most virulent viruses known, with a very high fatality rate
But Virology Institute of the CCP still deliberately isolated these viruses from bats
The fifteen papers selected in these tweets are evidence that the Virology Institutes of the CCP detected and isolated viruses from various animals.
The P4 laboratory of the CCP is looking for viruses in all kinds of animals from all over the world.
Some viruses were isolated, and some were only detected. But what was the purpose of the detection? Isn’t it just to see if there is something worth isolating?
These papers contribute zero to science because beyond simple detection and isolation of viruses from animals there are no discoveries of new mechanisms.
Although they can say that this is to study the evolution of viruses, they haven’t done any in-depth research, apart from detecting and isolating these viruses and doing the simple classification analysis that an undergraduate student can do.
After the virus was detected or isolated, they did not deeply study the molecular mechanism of virus evolution, nor did they search for the targets of vaccines, antibodies, and drugs from virus evolution for antiviral research.
What they did is just to detect and isolate various viruses one by one from various animals that can pose a huge potential danger to humans (especially after laboratory transformation!)
These viruses, I believe, will become options in the bio weapon stockpile in this research institute controlled by the Chinese military.
The danger will be inestimable, if these viruses are modified and recombined in the hands of the Communist China military.
Also, these are just a few of the published papers. The military also did separations and modifications of viruses. How many were unpublished??!
The CCP old hands in Zhongnanhai must be held responsible for this!
In the third article, we described the process of making a viral genetic weapon1). Discover the virus. 2). Recombination techniques. 3). Recombinant virus creation and whitewashing. 4). Animal model testing. This process also revealed the necessary conditions for the mass production of viral biochemical weapons: 1. Live virus strain reserve 2. Personnel with recombinant virus technology mastery 3. Hardware for researching and experimenting recombination technology, include P3, P4 laboratories and various viral recombination and whitewashing technology 4. Animal laboratories and animals for animal model testing.
So let’s take a look at Communist China’s viral bioweapon research, development and production capabilities from these four aspects.
Before introducing these four parts, let’s take a look at the difference between viruses and bacteria:
Bacteria and viruses can make you sick, this is what they have in common. So what are the differences?
Firstly it is the difference in size: bacteria are much larger than viruses. Dr. Clemens Farich explained: “Bacteria can be a hundred times larger than viruses.” Most bacteria are about 0.6 to 1.0 microns (micrometers) in diameter, visible with an ordinary light microscopy. The much smaller viruses can only be seen under an electron microscope.
Secondly it is the difference in structure: the virus is simpler in structure. Farich said: “The main difference is the composition.” The difference between viruses and bacteria is their “anatomy”. The internal medicine expert explained: “For example, bacteria have cell walls and corresponding internal structures.” The cell walls contain cytoplasm, ribosomes, and genome, which is the origin of virulence.
The cytoplasm is the basic structure in which cells undergo metabolic processes. Ribosomes help protein synthesis and bacterial reproduction. In addition, many bacteria have one or more flagella for movement. Some species have so-called cilia, which facilitate attachment to the surface of other bacteria or cells.
Viruses are simpler in structure. They usually only have their own genetic material enclosed in a protein shell or capsid.
That means the viruses need a host to spread..
The proliferation of bacterial cells, like human cells, generally occurs through cell division. Bacteria cells copy their genetic makeup before dividing, and then contract in the middle of it, becoming two fully developed cells, which can become two new cells.
Viruses cannot replicate by themselves because they have no cytoplasm or ribosomes to replicate genetic material by themselves and self-repair. Therefore, viruses infect foreign cells, so-called host cells, and inject their own genetic information into them. Therefore, the virus reprograms the genetic information in the host cell genome to make it produce more viruses. The new virus leaves the host cell either by extracellular secretion (exocytosis), that is, the virus is excreted from the cell or by budding or lysis (apoptosis). During viral budding, viruses squeeze host cells; in lysis, the membrane of the host cell is lysed.
Bacteria are regarded as living organisms, while viruses are not. The bacterial life cycle and metabolism varies. Some bacteria need oxygen for metabolism, for others oxygen is toxic. Some bacteria require light to survive, or require other specific chemicals, such as sulfur. Viruses have no metabolism of their own and are therefore not considered living organisms!
Bacteria and viruses make us sick in different ways. Viruses can destroy cells in our bodies through proliferation, or triggering human immune cells to eliminate infected cells. Bacteria can make you sick, for example through their metabolites. In a way, they are human poisons.
This means that antibiotics are only effective against bacterial infections. Farich said: “Antibiotics affect and capture the structure of bacteria, such as the cell wall, which can lead to the death of bacteria.” Because the structure of the bacterial cell wall is different from that of the human cell membrane, antibiotics only attack the bacteria and not the human cells. Some antibiotics do not kill bacteria, but just prevent them from multiplying. Falich believes: “However, bacteria are independent cells, which can adapt to different environmental conditions. Therefore, bacteria can develop resistance to antibiotics.”
Antibiotics have no effect on viruses as they do not have their own metabolism or cell walls. However, there are drugs that can inhibit the proliferation of viruses. In many viral infections, such as the flu, the treatment does not target the virus itself, but can reduce the symptoms of the disease. The rest is left to the body’s own defenses. However, there are ways to target the virus itself. Antiviral drugs have different mechanisms to inhibit viral proliferation and are used in certain viral infections. The medical expert said: “They can prevent the spread of the virus in the body, but a drug that kills the virus without harming the body’s own cells has not yet been discovered.”
Antiviral drugs have different points of attack (targets) at different reproductive stages of the virus. Some drugs prevent the virus from entering or infiltrating the host cells, and other drugs interfere with the transcription or the translation process, or interfere with the capsid.
Vaccination protects against some bacterial and viral diseases.
Vaccines prepare the immune system against pathogens. Some vaccines target certain bacteria and viruses. Vaccines could contain diluted pathogens or only part of a pathogen that is harmless. The immune system recognizes these foreign structures and produces antibodies against them. If the corresponding pathogen entered a vaccinated host, the invasion often quickly fails. Falich said: “Through the decades, vaccination against many viral diseases such as polio were achieved, but HIV and hepatitis C vaccination has been unsuccessful due to the complex characteristics of these viruses.”
In addition, some viruses change their appearance very quickly, which makes it difficult to develop an effective vaccine. Therefore, for example, a new influenza vaccine must be developed every year.
After clarifying the difference between viruses and bacteria, we list the pathogen resources that the CCP has at its disposal.
Part 1: Live virus strains
In 2009, Chen Zhu, then Minister of Health, issued the Ministry of Health Order No. 68, promulgating the <Administrative Measures for the Management of depository institutions for Pathogenic Bacteria (viruses) transmissible in Humans>. Since then pathogenic bacteria (viruses) transmitted by humans have been reserved as a national strategic combat readiness resource. Let’s first have a look at the contents of the administration measures.Contents of this administration protocol PDF
List of members of the National Health and Family Planning Commission
Microbiological Pathogen Laboratory Biosafety Review Expert Committee
(February 2016)List of members of the National Health PDF
This table lists the personnel and their source organizations and specific divisions of labour of the “National Pathogenic Microbiology Laboratory Biosafety Experts Committee” responsible for the safety assessment, technical consultation and demonstration of the depository institutions. This table also shows the deposit and management mode of viruses and bacteria. Viruses and bacteria should be preserved and managed in laboratories. In this table, you can see that the main laboratories of the depository institutions are the laboratories of the regional CDCs under the nation CDC, including the laboratories of various medical research departments. The most important P4 laboratory is not included in this table, because the level of this committee is not high enough. Of course, even depositories of this level must abide by strict confidentiality regulations, because these viruses and bacteria resources, like laboratories, are properties of CCP. They are nothing but custodians.
In the construction of the “Knowledge Innovation Project” of the Chinese Academy of Sciences, one of Chen Zhu’s top ten biomedical platforms, viruses and bacteria resources platforms were included. So viruses and bacteria were integrated into two major platforms: The National Pathogen Microorganism Resource Bank and the National Microbiology Species Resource Bank.
The National Pathogen Microbiology Resource Bank is composed of four deposit centers: the Institute of Microbiology of Chinese Academy of Sciences, Institute of Infectious Diseases of Chinese Center for Disease Control and Prevention, Shanghai Institute of Plant Physiology and Ecology of Chinese Academy of Sciences, and Institute of Oceanology of Chinese Academy of Sciences. The Pathogenic Microorganism Depository of the Institute of Microbiology of Chinese Academy of Science forms the core component. It was transferred and integrated into the Wuhan Institute of Virus Research after SARS in 2003. Pathogenic microorganisms generally refer to viruses and bacteria that can cause disease.
The core of the National Microbiology Species Resource Bank is 10 national-level microorganism species deposit centers, including the Chinese Microorganism Species Deposit Center, the National Agricultural Microorganism Species Deposit and Management Center, the Chinese Medical Microorganism Species Deposit and Management Center, the Chinese Pharmaceutical Microorganism Species Deposit and Management Center, the Chinese Industrial Microorganism Species Deposit and Management Center, the Chinese Veterinary Microorganism Species Deposit and Management Center, Chinese General Microorganism Species Deposit and Management Center, Chinese Forestry Microorganism Species Deposit and Management Center, China Marine Microorganism Species Deposit and Management Center, China Typical Culture Deposit and Management Center, integrating the model strains in nine fields including agriculture, forestry, medicine, pharmacy, industry, veterinary medicine, marine, fundamental research, and educational experiment, as well as strain resources with important or potential application value. As of 2018, the platform’s total storage resources reached 235,070 strains, with more than 3.2 million back-up copies. Among them, 150,177 strains can be shared externally, belonging to 2,484 genera and 13,373 species, accounting for about 80% of the total domestic sharable resources. The resource possession ranks first globally amongst microbiological depositories, containing quality microorganism species resourced used in domestic microbial fertilizers, microbial feed, microbial pesticides, microbial environment treatment, edible fungus cultivation, food fermentation, biochemical industry, product quality control, environmental monitoring, vaccine production, drug development, etc. At the same time, it also preserves a wide variety of standard and model microorganism species for basic research in life sciences. In recent years, the Microorganism Species Resource Bank has paid more attention to the collection of microorganisms from special habitats, including the world’s three poles (Antarctic, Arctic, and Tibetan Plateau), deep ocean, desert, saline and other environments. At present, there are about 6,700 polar microbial resources and more than 23,000 marine microbial resources, and the largest marine microbial species stock in the world.
According to official reports:
On April 27, 2018, the reporter learned from the Wuhan Branch of the Chinese Academy of Sciences that the National Health Commission has officially designated the Wuhan Virology Institute Microorganism (Poison) Species and Samples Deposit Center as the “National Level Deposit Center” (hereinafter referred to as the “Center”). It is understood that the center was established in 1979 and registered in 1989 in the World Federation of Culture Collection Committee. The Center joined the Global Alliance of European Virus Resource Banks in 2015 and， in October 2017， successfully passed the highest-level assessment of the Alliance’s quality management system. It is a composite research center amalgamating the collection and preservation of virus resources, virus biotechnology research and development, system virology and bioinformatics research. The Center is also the largest virus collection center in Asia. About 1500 strains of various types of virus isolates are preserved, covering 1/3 of the virus families that have been released worldwide. The live virus stored in the library covers human medical viruses, zoonotic viruses, animal viruses, insect viruses, plant viruses, bacteriophages, environmental microorganisms, virus-sensitive cell banks, and virus genetic resource banks. Viruses with names that strike fear into hearts, such as Zika virus and Xinjiang Hemorrhagic Fever virus, are amongst these.
It may be misunderstood that this deposit center only stores about 1500 strains of various virus isolates, by merely looking at this article and the publicly available information, but this actually exposes the evidence of a cover-up. The fact it was named “China General Virus Deposit Center” also points to a deliberate cover-up. The Center does not preserve general viruses, but killer viruses that can be weaponized. The director of the center is Hu Zhihong. Hu Zhihong is currently the director of the China General Virus Deposit Center and the director of the Microorganism Resources and Application Center, Wuhan Institute of Virology. Hu was the former director of the Wuhan Institute of Virology and the former deputy director of the State Key Laboratory of Virology. Researcher Deng Fei is responsible for the detailed management and construction of this deposit center. Judging from the name “China General Virus Deposit Center” and the number of concealed and preserved viruses, the virus preservation here is strictly classified, and some virus preservation is not done by Wuhan Virus Research Institute.
Let’s have a look at where this virus deposit center is. From the above article, it is clear that the main office address and laboratory of Wuhan Virus Institute is in Xiaohongshan instead of Zhengdian. Zhengdian is where the P4 laboratory is located, with only 28 research staff. Then this depository center must be in Xiaohongshan instead of Zhengdian. The following report of Chen Zhu’s inspection of Wuhan Virology Institute in 2014 proves this point.
In this photo, Chen Zhu was listening to a report on a project in Zhengdian (P4)
Subsequently, Chen Zhu and his entourage visited the Molecular Virology and Biological Engineering Technology Discipline Group, Novel Virus Science Group, Virus Deposit Center and Analysis and Test Center amongst others. Chen Zhu expressed his affirmation on the work of virus research, resource preservation and public technical service platform construction of Wuhan Virus. During the visit, Chen Zhu had a cordial conversation with young scientists to understand their subject areas and research directions, and encouraged young scientists to concentrate on research, focus on innovation and creativity, and promote the development of China’s biological, medical, and health undertakings.
Let’s take a look at how many viruses are stored in the virus deposit center of Wuhan Virus Research Institute. We pull out the data in the P4 laboratory operation report released in 2018: “Live viruses, as a very important resource, must be collected and preserved strictly to provide the strategic reserve necessary for scientific research. The China and Africa Research Center for Microbiology and Epidemic Control, the construction of which was led by the Wuhan Virus Research Institute, has identified a number of bat, rat, and camel-derived pathogens in Kenya. In other countries along the One Belt and One Road, such as Pakistan, tasks to separate, identify, prevent and control new contagious pathogens such as Chikungunya Fever were deployed.
The Xinjiang hemorrhagic fever virus may cause great harm, and it is also the only serious pathogen of natural epidemic sources found in China. The research team led by Hu Zhihong and Deng Fei has traveled to remote areas such as Xinjiang many times, collected more than 50,000 tick samples, and isolated three strains of Xinjiang hemorrhagic fever virus from Hyalomma Asiaticum ticks with method of inoculation by suckling mice.
The Microorganism Species Resources and Applications Center of Wuhan Institute of Virology has established a pathogenic microorganism resource and information platform. It is currently the largest live virus deposit center in Asia. At present, there are more than 1,400 types of virus species deposited and more than 60,000 strains. It is a core member of the largest international union of virus resource depository institutes “European Virus Resource Depository: Going Global”. The two Zika virus strains brought from France through this union provides strong support for Zika virus research in China. Please note that there are more than 1,400 species and more than 60,000 strains! The “Chinese Basic Virus Resource Bank” built on this basis also proves this, and the key manager of this “Chinese Basic Virus Resource Depository” is Deng Fei.
Jan 2018-present: Director of the Center for the Collection of Microorganism Species and Samples, Wuhan Institute of Virology, Chinese Academy of Sciences
Sep 2013-present: Researcher, Group Leader, Wuhan Institute of Virology, Chinese Academy of Sciences
Dec 2012-present: Deputy Director of the Center for Microorganism Poison Resources and Applications, Wuhan Institute of Virology, Chinese Academy of Sciences
Sep 2007-Aug 2013: Associate Researcher, Wuhan Institute of Virology, Chinese Academy of Sciences
Jul 2003-Sep2007: Assistant Researcher, Wuhan Institute of Virology, Chinese Academy of Sciences
April 2002-Oct 2002: Visiting scholar, Department of Virology, Wageningen University, Netherlands
Jan 2001-Apr 2001: Visiting scholar, Great Lakes Forestry Center, Canada
Research Projects (limited to 10)
Based on the projects hosted by Deng Fei, from the collection and preservation of virus strains to the construction of data management and database systems, she has participated and played a leading role.
Query result: Total 1022 records
Animal virus 666 records
Medical viruses 90 records
Plant viruses 74 records
Insect virus 203 records
Bacterial viruses 68 records
Insect virus 203 records
Search coronavirus 44 related records
By searching and displaying the contents of this database, we know that the basic classification of deposited viruses has been completed. There are indeed only 1,400 numbered deposited strains that can be searched. This confirms the judgement of DT excavator, of over 60,000 strains, only 1,500 isolated strains have been deposited in the China General Virus Collection Center in Xiaohongshan, while the other nearly 60,000 strains are deposited in the P4 laboratory in Zhengdian. Virus strains transferred after 2003 from the Institute of Microbiology, Chinese Academy of Sciences are among them. More than 1,400 species and 60,000 strains, this is the real strength of the P4 laboratory.
Let’s take a look at a report to see how these deposited viruses are used.
Wuhan Virus Institute Release Time 2018-04-28
Yangtze River Business News (reporter Zhang Heng correspondent Chen Zuzhaoyu Wang Yihao) 27, the reporter learned from the Chinese Academy of Sciences, Wuhan Branch, the National Health Commission has officially designated the Wuhan Virus Institute of microbial bacteria (viruses) species collection center for the “National Collection Center” (hereinafter referred to as “Center”), the future will be oriented to national strategic needs, in national security, life sciences research, population health and virology research areas to play an important and indispensable support role.
It is known that the Center was founded in 1979 and registered with the International Committee for the Preservation of Microbiological Species in 1989. It joined Europe Virus Repository Global Alliance in 2015, and successfully passed the highest level of the Alliance’s quality management system rating in October 2017. It has become a comprehensive research center integrating virus resource collection and preservation, virus biotechnology research and development, phylogenetic virology and bioinformatics research. It is also the largest virus preservation center in Asia.
Recently, Wuhan Institute of Virology, Chinese Academy of Sciences, has officially become one of the four “National Collection Centers” in China. This means that the Center is qualified to preserve the most dangerous viral pathogens, such as Ebola and Nipah, for research activities.
It is home to the largest virus repository in Asia, containing more than 1,500 isolated viruses, covering one third of all the released viruses worldwide including Zika, Xinjiang hemorrhagic fever and other strains of viruses.
On May 16, a reporter of Chutian Metropolis Daily visited the virus library and witnessed the amazing journey of a virus from “waking” to “sleeping”.
The reporter also witnessed the virus sample isolation.
“People are generally afraid when it comes to viruses, and that’s because they don’t understand them,” Deng Fei, deputy director of the preservation center of the Wuhan Institute of Virology, Chinese Academy of Sciences, said. Professionally trained lab technicians, although dealing with viruses every day, can safely carry out operations on non-violent viruses under the protection of strict measures.
On May 16, a report from Chutian Metropolis was granted access to the P2 virus sample isolation chamber. P2 requires a relatively low level of protection, but it is still “tightly guarded”. After watching the demonstration of how to put on protection gears by lab technician Wu Qiaoli, the reporter put on a long-sleeved lab coat, a blue protective suit, a mask, a hat, shoe covers, a pair of shoes, latex gloves, plastic film gloves, etc. “These are special disposable protective gear, and the requirement is to expose skin as little as possible,” Wu Qiaoli said.
The lab technician, Zhang Yanfang, was isolating the Zika virus. She sat in front of a biosafety cabinet, her arm operating inside the cabinet and the rest of her body outside, her eyes staring through a transparent hood at the Pipette, putting the purple-red Zika virus into separate culture flasks for cell culture. “Purplish red is the color of the indicator. The viruses are the smallest organisms at the nanoscale and have no color, and we add special fluorescent dyes so we can identify their presence,” Zhang Yanfang said. She paded the operating table with absorbent towels beforehand so that if there is a sample leak, it can be disposed of immediately.
Whether pipetting or culturing, Zhang Yanfang’s movements are gentle. “The operation process may produce aerosols, which are invisible and can be infection once people touch them, so you have to be very careful.” She said. The reporter noticed that there is a device similar to a hood above the safety cabinet, pumping the airflow inward, so any small gas reaction can’t get out! Safe Cabinet.
Various Viruses Slumber at Different Temperatures
The mysterious virus vault has two doors, with a “biohazard” warning sign on the outer electronic door.
Compared to the stifling heat outside, the inside of the vault is cooler. The room, which is less than 100 square meters, is divided into four smaller rooms, one of which has a large capacity refrigerator. The other three rooms are -40 ℃, -80 ℃, -150 ℃ virus storage room, each room has several neatly placed refrigerators. A large lock is attached to every refrigerator.
Deng Fei introduced that the windows of the storage room are equipped with infrared anti-theft systems. In order to enter the warehouse, open the refrigerator, there must be at least two people present, perform complete registration procedures.
Zhang Yanfang wearing special anti-freeze gloves, opened a refrigerator displaying real-time temperature of -77 ℃. She introduced, each refrigerator has a transmitter to connect to the office computer, for remote monitoring refrigerator temperature changes, and there is a backup power in a power security.
The reporter saw that the refrigerator had 4 small doors inside. Zhang Yanfang opened one of the doors, which is arranged in 25 small stainless steel drawers. She pulled a drawer, inside which was a plastic box with a number of virus freeze-storage tubes, some were blue, some were red, the tube body was labelled with a number and QR code. “These numbers are on file, you can find out where the virus you need is in detail with a search on the computer,” Deng Fei said. These tiny frozen storage tubes, which house viruses such as hepatitis, Zika, influenza and herpes, sound a little chilling to outsiders, but tfor the researchers here, they are useful biological resources.
The “life and death cycle” keeps the virus alive.
“We work with agriculture, forestry and health systems and receive many types of pathology samples, including blood, urine, feces, pharyngeal Swabs, cerebrospinal fluid, herpes fluid, etc.” Deng Fei described. The work before the virus preservation is very complex, from receiving the pathology sample to performing tests, isolating the sample, cryopreservation, the virus goes from “awake” To “sleep” is a process of continuous refinement.
When the test points to certain viruses, the lab technician purifies them for culture. “Ninety-nine percent of microorganisms cannot be cultured; viruses belong to the remaining 1 percent. They can’t exist alone, but they can exist through cell culture,” Deng Fei explained. After the virus invades the cell through the cell membrane, it will continue to reproduce itself, with higher and higher titers. Only standardized viruses with a clear background and the highest titer can enter the repository. Each virus strain has multiple backups that are traceable.
Most of the -40°C refrigerator are used to keep disease material. Viruses are mostly kept in a -80°C refrigerator, which maintains a high level of activity for a period of time. Once the virus is not active enough, it is “revived”, i.e., the virus is inoculated into fresh cells to initiate a new round of infection. Virus numbers and activity return to a high level state and then are preserved.
In a 60°C environment, the virus will die in a few minutes. According to Deng Fei, bringing a virus back from dormancy is like a “life and death cycle”. The activation of each virus requires a corresponding culture environment. For example, Zika virus, it can infect monkey cells, only in a 37 ℃ incubator; while it can infect mosquito cells only in an incubator at 28°C.
The “oldest” strain is in its 30s.
Deng Fei introduces, the microbial (viral) species collection center at the Wuhan Institute of Virology, Chinese Academy of Sciences, has the largest virus repository in Asia, currently preserves over 1,500 isolated viruses, covering one-third of the world’s released virus families, including human medical viruses. The oldest strains of the virus are more than 30 years old, and the most common are the zoonotic, animal, insect and plant viruses. Among them, the oldest strain of virus is more than 30 years old.
“The Xinjiang hemorrhagic fever virus preserved here is the only class IV virus in China. Like the Zika virus, we have imported Asian, European and American strains, isolated and cultured seed batches, working batches, etc., and not only have we preserved the resources and also performed research,” Deng Fei said. The center was promoted to “national brand”, not only with the construction of biosafety level 4 laboratory in Han, but also in accordance with the national relevant Provisions for the receipt, testing, centralized storage and management of bacterial (virulent) strains or samples to entities legally engaged in experimental activities on pathogenic microorganisms. Provision of bacteriological (virulent) strains or samples and issuance of national standard bacteriological (virulent) strain certificates, and playing a greater role in technical research, operational training, etc.
“After more than 30 years, the accumulated virus resources have provided a strong guarantee for research work. Especially in the last few years, the preservation center has grown rapidly, with one to two hundred new viruses being preserved every year. Each virus strain is a treasure, and although some of them are of no practical use at the moment, they will be awakened and put to use in the future once there is a demand for them! .” Deng Fei said.
With this article we have a basic understanding of some of the details of virus vaulting and the use of vaults to preserve viruses.
A. How viruses are kept in a refrigerator in a preservation room, which is built in a preservation vault. That is, there can be more than one vault room in the vault, and the vault room is usually set up at a level. Preservation vaults have three gates and are cooler inside the vault. The storage room of less than 100 square meters is divided into four small rooms, one of which houses a large capacity refrigerator. The other three rooms are -40 ℃, -80 ℃, -150 ℃ virus storage rooms, each room has several neatly placed refrigerators. The refrigerators are where the strains of the virus are kept. All refrigerators have a large lock on them. The windows of the vault are equipped with an infrared security system. At least two staff members must be present to enter the vault and open the refrigerators, and they must follow the proper registration procedures. Each refrigerator has a transmitter connected to the office computer, which can remotely monitor changes in refrigerator temperature, and there is a backup power supply to ensure timely security. One of the refrigerators has four small doors inside. Zhang Yanfang opens one of the doors and there are 25 small stainless steel drawers arranged inside. She pulls open a drawer, plastic container box arranged in a number of virus frozen storage tubes, some were blue, some were red, the tube body labeled with a number and QR codes. “These numbers are on file, and what strain of virus you need to take, you can find out where it is in detail with a computer search,” Dunphy said. These tiny frozen storage tubes, which house viruses such as hepatitis, Zika, influenza and herpes, sound a little chilling to outsiders, but to the For researchers here, they are useful biological resources.
B. How are the viruses preserved? The virus is preserved by 1. receiving a pathological sample 2. testing 3. isolating from the sample 4. keeping it cool. These four processes are designed to move the virus from “awake” to “asleep” in a preserved state. The detection and isolation of the virus is done in a P2 class virus sample isolation chamber. Only standardized viruses with a clear background and the highest titer are accepted into the repository. Each virus strain has multiple backups and is clearly traceable. From receiving the pathology sample to testing, isolation and cryogenic freezing, the virus goes from “awake” to “asleep” in a continuous process. Refinement of the process.
Based on Deng Fei’s description, not only do we have a clear picture of how the virus is preserved and how it can be preserved, but we are also clear about the fact that it is simply not possible to preserve 60,000 strains of the virus here.
Let’s look at the latest information from P4 Labs in 2020.
What exactly is this article, published on March 31, 2020, trying to prove? Is the P4 lab safe or are people being told that the 60,000 strains aren’t here? The floor area of the P4 lab building and the investment has been a secret so far. But from Chen Zhu examining that photo, we know for sure that it must be huge, huge enough to hold the 60,000 plants Viral strains.
NEXT DT EXPOSÉ OF CHINESE P4 BIOSAFETY LABORATORY – SEASON 2 (10) (2)
Edited by 【Himalaya Hawk Squad】